Miscellaneous Questions- Part-1

Miscellaneous Questions

Q1. Confirmation of menstrual blood stain is done by the following method:

A)   Fibrin Degradation Product (FDP)

B)   Protein marker

C)   Restriction enzyme

D)   Iso enzyme marker

Answer: FDP (Fibrin Degradation Product)

Explanation: Menstrual blood is a complex biological fluid composed of blood,vaginal secretion and the endometrial cell of the uterine cell of the uterine wall.Menstrual blood is not highly oxygenated,its darker than normal blood.The differentiation is based on the composition of each blood, the content of hemoglobin,RBC,WBC is more in normal blood as compared to menstrual blood,as it contain dead and no longer functional tissues.

 

Q2. Arrange the test methods of blood in the proper sequence:

A)   Benzidine

B)   Absorption elution

C)   Visual examination

D)   Takayama crystal test

 Choose from the given option:

1.)  C),A),D),B)

2.)  A),C),D),B)

3.)  A),B),C),D)

4.)  D),C),A),B)

Answer: C),A),D),B)

Q3. The following are red cell enzymes except:

A)   Phosphoglucomutase

B)   Haptoglobin

C)   Adenylase kinase

D)   Peroxidase

Answer: Haptoglobin

Explanation: Haptoglobin is the protein that in humans is encoded by the HP gene and is produced by the liver.In blood plasma,haptoglobin binds with high affinity to free hemoglobin released from erythrocytes and inhibits its deleterious oxidative activity.The word “ haptein” refers “to bind” and hemoglobin (HB), this word is of Greek origin.

 

Q4. Which of the following is not used for nuclear DNA profiling?

A)   Plucked hair

B)   Kinky hair

C)   Fallen hair

D)   Cut hair

Answer: Cut hair

Explanation: Nuclear DNA is known as the molecule of life and contains the genetic instruction.Hair that has been cut does not contain DNA,since DNA is only found in live hair follicles at the root of a strand of hair where the surface of skin is found which has nucleated cells.

 

Q5. Assertion (A) : pH of blood is normally alkaline in living people but after death pH of blood changes.

Reason (R): Because of the accumulation of carbon dioxide from glycogenolysis and glycolysis.

A)   Both A and R are false

B)   Both A and R are true but R is not the correct explanation of A

C)   A is true but R is false

D)   A is false but R is true

Answer: A is true but R is false

 

Q6. Which of the following changes in a dead body does not help to determine time since death?

A)   Marbling

B)   Cadaveric spasm

C)   Hypostasis

D)   Foaming

Answer: Cadaveric spasm

Explanation: Cadaveric spasm is a condition in which a group of muscles that were used profusely just before death become stiff and rigid immediately after death.This instantaneous rigor mostly involves hands,very rarely the entire body. For eg in cases of assaults,suicides,drowning,the victim hand present as rigid and clenched,holding/grasping onto the clothing,buttons,or hair of the assialant.The cadaveric spasm is valuable in connecting on the manner of death,not the time since death.

 

Q7. Vaginal secretion can be characterized on the basis of the following:

A)   Squamous cells

B)   Elongated cells

C)   Papillary cells

D)   Glycogenated epithelial cells

Answer: Squamous cell

Explanation: The vaginal epithelium is the inner lining of the vagina consisting of multiple layers of squamous cells.Parabasal cells are the smallest epithelial cells which are derived from the basal layer of squamous epithelial cells.They are round and responsible for the vaginal secretion.

Q8. Match the following

A)   Jute                          1) Mineral fiber

B)   Cotton                      2) Seed fiber

C)   Asbestos                   3) Leaf fiber

D)   Sisal                          4) Bast fiber

  Choose the correct option:

A)   A- 4, B- 2, C - 1,D-3

B)   A- 4, B - 2, C- 3,D-1

C)   A - 1,B - 3,C - 4,D-2

D)   A - 1,B - 4,C - 3,D-2

Answer: A) A-4,B-2,C-1,D-3

Q9. A technique used to separate sperm cells and non sperm cells for DNA analysis is called as:

A)   Qi Amp extraction

B)   Chelex extraction

C)   Differential extraction

D)   Organic extraction

Answer: Differential extraction

Explanation: Differential extraction also known as differential lysis refers to the process by which DNA from two different types of cells can be extracted without mixing their contents.The common application of this method of extraction is separation of vaginal epithelial cells and sperm cells.

 

Q10. In PCR reaction the annealing temperature depends on:

A)   AT contents of sequence

B)   Primer dimer

C)   Concentration of Mg used in reaction

D)   GC content in sequence

Answer: GC content in sequence

Explanation: The annealing temperature is that temperature at which the primers anneal or attach to the template.When we separate the two strands at high temperature,the temperature must be go down so that the primer binds to template to form stable H- bond ( on colling). G and C residue in both primer and template play a big role in deciding what temperature will be best for the mix i.e. it determines melting temperature or Tm  of the primer.

 

Author:- Tashi Bhargawa