Q1. Which type of DNA polymorphism is analyzed in mitochondrial DNA (mtDNA) typing?
a) SNP (Single Nucleotide Polymorphism)
b) STR (Short Tandem Repeat)
c) VNTR (Variable Number Tandem Repeat)
d) RFLP (Restriction Fragment Length Polymorphism)
Answer: a) SNP (Single Nucleotide Polymorphism)
Explanation: Mitochondrial DNA (mtDNA) analysis often focuses on SNPs
due to their high mutation rate and maternal inheritance pattern, which makes
them useful in forensic investigations.
Q2. In forensic toxicology, which analytical technique is
most appropriate for detecting and quantifying trace levels of drugs and
poisons in biological samples?
a) High-Performance Liquid Chromatography (HPLC)
b) Gas Chromatography-Mass Spectrometry (GC-MS)
c) Ultraviolet Spectroscopy
d) Thin Layer Chromatography (TLC)
Answer: b) Gas Chromatography-Mass Spectrometry (GC-MS)
Explanation: GC-MS is highly sensitive and specific, making it the
preferred method for detecting and quantifying trace levels of drugs and
poisons in forensic toxicology.
Q3. Which of the following is a hallmark of a manual
strangulation homicide?
a) Petechial hemorrhages
b) Hyoid bone fracture
c) Defensive wounds on the hands
d) Ligature marks on the neck
Answer: b) Hyoid bone fracture
Explanation: A fractured hyoid bone is a common indicator of manual
strangulation, while petechial hemorrhages can occur in various types of
asphyxia.
Q4. The Kastle-Meyer test is used to detect the presence
of:
a) Saliva
b) Blood
c) Semen
d) Urine
Answer: b) Blood
Explanation: The Kastle-Meyer test is a presumptive blood test that uses
phenolphthalein and hydrogen peroxide to detect the presence of hemoglobin.
Q5. What is the significance of the Becke line test in
forensic science?
a) To determine the blood type of a sample
b) To analyze the chemical composition of a drug
c) To assess the refractive index of glass
d) To identify the presence of semen
Answer: c) To assess the refractive index of glass
Explanation: The Becke line test is used to determine the refractive
index of glass fragments, which can help match them to a source.
Q6. In forensic entomology, the term "postmortem
interval" refers to:
a) The time elapsed since death
b) The age of insect larvae found on a corpse
c) The time taken for decomposition to start
d) The period between death and discovery of the body
Answer: a) The time elapsed since death
Explanation: Postmortem interval (PMI) is the time that has passed since
a person has died, often estimated using insect development stages.
Q7. Which substance is used as a matrix in
Matrix-Assisted Laser Desorption/Ionization (MALDI) mass spectrometry?
a) Acrylamide
b) Sinapinic acid
c) Silver nitrate
d) Coomassie Brilliant Blue
Answer: b) Sinapinic acid
Explanation: Sinapinic acid is commonly used as a matrix in MALDI-MS to
facilitate the ionization and detection of large biomolecules.
Q8. Which of the following is considered a confirmatory
test for the presence of blood?
a) Luminol test
b) Leucomalachite green test
c) Takayama test
d) Kastle-Meyer test
Answer: c) Takayama test
Explanation: The Takayama test is a confirmatory test for blood, as it
produces specific hemochromogen crystals when blood is present.
Q9. The term "medullary index" in hair analysis
refers to:
a) The ratio of the medulla's diameter to the entire
hair's diameter
b) The percentage of keratin in the hair shaft
c) The number of cuticle scales per inch of hair
d) The length of the hair shaft
Answer: a) The ratio of the medulla's diameter to the entire
hair's diameter
Explanation: The medullary index is used to differentiate between human
and animal hair based on the relative size of the medulla.
Q10. Which of the following is a key characteristic of a
contact gunshot wound?
a) Abrasion collar
b) Soot deposition inside the wound
c) Tattooing around the wound
d) Stellate tears
Answer: b) Soot deposition inside the wound
Explanation: A contact gunshot wound often exhibits soot deposition and
powder burns inside the wound due to the close proximity of the firearm.
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