1. In STR-based forensic DNA profiling, what is the most likely source of allele drop-out in low template DNA samples?
A) Contamination during PCR setup
B) High number of STR loci
C) Stochastic effects during amplification
D) Use of fluorescent dyes
Answer: C) Stochastic effects during
amplification
Explanation: Low-template DNA can result in
allele drop-out due to stochastic variation during PCR. This leads to failure
of one allele (especially in heterozygotes) to amplify, complicating
interpretation.
2. Which statistical method is most
appropriate for evaluating the match probability in mixed DNA profiles
involving multiple contributors?
A) Hardy-Weinberg Equilibrium
B) Likelihood Ratio
C) Chi-Square Test
D) Standard Deviation
Answer: B) Likelihood Ratio
Explanation: The likelihood ratio approach
quantitatively evaluates competing hypotheses (e.g., inclusion vs. exclusion)
in complex DNA mixtures, incorporating population allele frequencies.
3. The identification of latent fingermarks
on adhesive surfaces like duct tape is optimally performed using:
A) Ninhydrin
B) Powder dusting
C) Cyanoacrylate fuming followed by
Rhodamine 6G
D) Silver nitrate
Answer: C) Cyanoacrylate fuming followed by
Rhodamine 6G
Explanation: On nonporous and adhesive
surfaces, cyanoacrylate fuming polymerizes on latent prints. Subsequent
staining with Rhodamine 6G enhances visibility under fluorescence.
4. Which of the following is NOT a
characteristic feature of diatom analysis in forensic drowning cases?
A) Resistance to putrefaction
B) Presence in cerebrospinal fluid confirms
drowning
C) Entry through gastrointestinal tract
postmortem
D) Siliceous cell wall structure
Answer: C) Entry through gastrointestinal
tract postmortem
Explanation: Diatoms enter circulation via
alveolar-capillary membranes during drowning, not through ingestion. Their
presence in bone marrow/CSF is confirmatory of ante-mortem inhalation.
5. Which of the following is most suitable
for detecting hydrocarbon accelerants in fire debris?
A) FTIR Spectroscopy
B) GC-FID with passive headspace extraction
C) TGA
D) UV-Visible Spectrophotometry
Answer: B) GC-FID with passive headspace
extraction
Explanation: Gas Chromatography-Flame Ionization Detection after passive headspace concentration is ideal for analyzing volatile hydrocarbons from charred debris in arson investigations.
6. The most reliable skeletal indicator of
sex in subadult human remains is:
A) Skull shape
B) Mandibular angle
C) Long bone length
D) Greater sciatic notch width
Answer: D) Greater sciatic notch width
Explanation: In subadults, sexual
dimorphism is subtle. However, the greater sciatic notch of the pelvis shows
early dimorphic traits, making it the most reliable among limited options.
7. Which of the following chemical
reactions is the basis for the Teichmann test for blood confirmation?
A) Formation of hemin chloride crystals
B) Reduction of hydrogen peroxide
C) Oxidation of phenolphthalin
D) Acid hydrolysis of hemoglobin
Answer: A) Formation of hemin chloride
crystals
Explanation: The Teichmann test detects
blood via formation of hemin crystals (ferriprotoporphyrin chloride) when
hemoglobin reacts with glacial acetic acid and salts.
8. In forensic handwriting analysis, the
term “line quality” refers to:
A) Thickness and darkness of the pen line
B) Speed, rhythm, and pressure control of
strokes
C) Spelling and punctuation
D) Type of ink used
Answer: B) Speed, rhythm, and pressure
control of strokes
Explanation: Line quality is a primary
handwriting feature involving naturalness, smoothness, and variation in stroke
pressure, aiding in distinguishing genuine from forged handwriting.
9. Which of the following best explains the
increased mitochondrial DNA (mtDNA) success rate in degraded forensic samples?
A) Its circular structure enables better
amplification
B) Higher mutation rate increases allele
variability
C) Its abundance per cell is significantly
higher than nuclear DNA
D) It undergoes less chemical degradation
than nuclear DNA
Answer: C) Its abundance per cell is
significantly higher than nuclear DNA
Explanation: mtDNA exists in thousands of
copies per cell, enhancing recovery from degraded or minimal biological
materials (e.g., hair shafts, bones).
10. Which method is best suited to
differentiate between genuine and forged ink entries made at different times on
the same document?
A) Oblique lighting
B) ESDA
C) Infrared Reflectance Spectroscopy
D) Thin Layer Chromatography
Answer: C) Infrared Reflectance
Spectroscopy
Explanation: Infrared reflectance can
detect chemical differences in ink, allowing visualization of additions or
alterations made at different times, especially with inks that are visually
similar.
Content Writer:- Srishti Dutta
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